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IDENTIFIERS
Sample ID: |
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TAXONOMY
Phylum | Arthropoda | Subfamily | |
Class | Insecta | Genus | Ceroplastes |
Order | Hemiptera | Species | Ceroplastes stellifer |
Family | Coccidae | Subspecies |
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SPECIMEN DETAILS
Life Stage | Brief Note | ||
Sex | |||
Tissue | |||
Voucher Status |
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COLLECTION DETAILS
Country | Date Collected: | ||
Province/State | Collectors | ||
Region/County | Elevation | ||
Sector | Elv. Accuracy | ||
Exact Site | Brief Note | ||
Latitude | |||
Longitude | |||
Coord Source | |||
Coord Accuracy |
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SEQUENCE: | COI | ||
Sequence ID | GenBank Accession | MK543895 | |
Last Updated | Genome | Mitochondrial genome | |
Locus | |||
Nucleotides | 543 bp | ||
ACAATAAATAATTATAACTTCAATTTAACATATTATATAATAATTACTATTCATGCATTATTAATAATTTTTTTTATAACAAT ACCAATCATTATTGGAATATTTAGAAATTGATTAATTCCACTAATAACATCAAGACAAGATTTAATCTATCCACGATTAAA TAATTTAAGAATATGAATTCTATTTCCATCATTAATAATTATAACAATAAGAATATTTATTAAAAATAAAATTTTTACAGGA TGAACCTTATATCCACCATTATCTATCCAAAATTCCGTATCAATTAATTTTACAATTTTATCACTACATTTAAACGGATTATC ATCAATTTTAAGATCTATAAACTTTATTATTTCAATACTAAATATAAATTCAAACAAAATATTTTTAAATAACTTATCACTAT ATTGCTGATCTATTATCATTACTTCAATACTAATTATTATTTCAATTCCAGTTTTAGCAAGAGGAATTACAATAATTATTATA GACCACAATTTTAACTCAATATTTTTTAATCCATTAGGAAATGGAAAT | |||
Amino Acids | |||
TMNNYNFNLTYYMMITIHALLMIFFMTMPIIIGMFSNWLIPLMTSSQDLIYPRLNNLSMWILFPSLMIMTMSMFIKNKIF TGWTLYPPLSIQNSVSINFTILSLHLNGLSSILSSMNFIISMLNMNSNKMFLNNLSLYCWSIIITSMLIIISIPVLASGITMIIM DHNFNSMFFNPLGNGN | |||
Illustrative Barcode | |||
 | |||
Length | Pcr Primers | Read(5’-3’) | Status | References |
543 bp | PcoF1/ HCO | Forward primer： CCTTCAACTAATCATAAAAATATYAG Reverse primer： TAAACTTCAGGGTGACCAAAAAATCA | high qual | 【1】【2】【3】 |
543 bp | PcoF1/ LepR1 | Forward primer： CCTTCAACTAATCATAAAAATATYAG Reverse primer：TAAACTTCAGGGTGACCAAAAAATCA | high qual | 【1】【2】【3】 |
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SEQUENCE: | 18S ribosomal RNA gene | ||
Sequence ID | GenBank Accession | MK533166 | |
Last Updated | Genome | ||
Locus | |||
Nucleotides | 602 bp | ||
GCCATGCATGTCTAAGTACAAGCTGTCCCGCACAGTGAAACTGCGAAAGGCTCATAAATCAGTTATGGTTCCTTAGATCGTAC CCACGTTACATGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACAGAGCTCCGACCAGCGCGTCGTCGTTCGGCGGTT CGCCCGTCGTCGTCGGCCGTTCGGAAGGAGCGCTTTTATTAGATCAAAACCGGCCAAGCGTTCGACGTGCGTTTCGCGCTCGG ACGTAAGTGGCCCGCGCAAAGACTTGACGACTCTGAATAACTTCGAGCTGATCGCACGGTCCTCGTACCGGCGACGTATCTTT CAAATGTCTGCCTTATCAACTGTCGACGGTAGGTTCTACGCCTACCGTGGTGGTAACGGGTAACGGGGAATCAGGGTTCGATT CCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCACGAAAATTATCCATTCAGGACTCCTGGAG ATAGTGACGAAAAATAACGATACGGGACTCATCCGAGGCCCCGTAATCGGAATGAGTACACTTTAAAACCTTGGTCACACGA GGATCAATTGGAGGGCAAGTCTGG | |||
Illustrative Barcode | |||
 | |||
Length | Pcr Primers | Read(5’-3’) | Status | References |
602 bp | 2880/ Br | Forward primer： CTGGTTGATCCTGCCAGTAG Reverse primer： CCGCGGCTGCTGGCACCAGA | high qual | 【1】【4】 |
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SEQUENCE: | 28S ribosomal RNA gene | ||
Sequence ID | GenBank Accession | MK533217 | |
Last Updated | Genome | ||
Locus | |||
Nucleotides | 757 bp | ||
AACCGTTCGGGGGTAAACGGACAGAGCCCGTGAATCCGGGCGCGCGGAATTCAGGACCGACGGCGGCCGTCGTCGATTCGA TATTTCCGTCGCCGACCCTCACCGGGTGCGGACGTCGCGACCCGTTCGCCGTCGATGCTTAGGGACGCGTTTTCGGTTCGCCA GCGCGGTCGCTTCGGCGGGCGACGACGGCGCGTTTCCGTTGTTTCGACGCGGACGGTAGCTTTGGCGCGGGTATTCGACGCG GCCTCGCCCGGCGCGTTTAGCGTCGGGCGGCGCTCGCGTCGACGCCGGTCAGCGACGAATCTTCGGGCCTCTTTCCGACCCGT CTTGAAACACGGACCAAGGAGTCTAGCGTGCGCGCGAGTCGCGGGGTATCCGAGCCGTCGCGCGGCTCGTCACGATACGAA ACCCGTGTACGTACGTTGTGCGAAGGCGTAATGAAAGTGAAAGGCGGCGCTTCGCGCGTCGCCGGGGAAGATGGCGTTCCGA GTCCACGTTTCGCGGTCGCACTGTACCGCGGCCGGACGCTCGCATTCCCAGGGCGTCCCGCTCGTACGTCGGCGGTCGGGGTT CGCTTCGGCCGCCGTCGAACTGGGGCGCACCTACAGCGTGCACGCTGGTACCCGAAAGATGGTGAACTATGCCCGGCCAGGA TGAAGTCAGGGGAAACCCTGATGGAGGTCCGCAGCGATTCTGACGTGCAAATCGATCGTCTGAGCTGGGTATAGGGGCGAAA GACTAATCGAACCATCTA | |||
Illustrative Barcode | |||
 | |||
Length | Pcr Primers | Read(5’-3’) | Status | References |
757 bp | S3660/ A335 | Forward primer： GAGAGTTMAASAGTACGTGAAAC Reverse primer： TCGGARGGAACCAGCTACTA | high qual | 【1】【5】【6】 |
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SEQUENCE: | EF-1α | ||
Sequence ID | GenBank Accession | MK547558 | |
Last Updated | Genome | ||
Locus | |||
Nucleotides | 525 bp | ||
ACCGGTGAATTCGAAGCTGGTATTTCGAAGAACGGACAGACTCGTGAGCACGCTTTGTTGGCTTTCACTCTGGGTGTAAGACAATT GATCGTCGGTGTGAACAAGATGGACTCTACCGAACCGCCATACAGCGAAGCTCGTTTCGAGGAAATCAAGAAGGAAGTAGCCAA TTATATTAAGAAGATCGGTTATAACCCAGCAACCGTCGCCTTCGTACCAATCTCCGGCTGGAACGGCGACAACATGTTGGAACCCT CCGACAAGATGCCATGGTTCAAGGGATGGGCGATCGAGCGTAAGGAAGGTAAAGCCGACGGTAAATGCCTGATCGAAGCTCTGG ACGCCATTTTGCCACCCAGCCGACCCACCGACAAACCTCTGCGTCTCCCACTTCAGGACGTGTACAAAATTGGTGGTATCGGTACT GTACCGGTTGGTCGAGTTGAAACTGGAGTCTTGAAACCCGGTATGGTCGTGACCTTCGCCCCCGCCAATTTGACCACTGAAGTTAA ATCCGTCGAAGCC | |||
Amino Acids | |||
TGEFEAGISKNGQTREHALLAFTLGVRQLIVGVNKMDSTEPPYSEARFEEIKKEVANYIKKIGYNPATVAFVPISGWNGDNMLEPS DKMPWFKGWAIERKEGKADGKCLIEALDAILPPSRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPANLTTEVK SVEA | |||
Illustrative Barcode | |||
 | |||
Length | Pcr Primers | Read(5’-3’) | Status | References |
525 bp | ScutA/ rcM52.6 | Forward primer： ATTGTCGCTGCTGGTACCGGTGAATT Reverse primer： GCYTCGTGGTGCATYTCSAC | high qual | 【1】【7】【8】 |
REFERENCES
[1] Choi, J., & Lee, S. (2019). Molecular phylogeny of the family Coccidae (Hemiptera, Coccomorpha), with a discussion of their waxy ovisacs. Systematic Entomology. doi:10.1111/syen.12404 [2] Park, D.S., Suh, S.J., Oh, H.W. & Hebert, P.D.N. (2010). Recovery of the mitochondrial COI barcode region in diverse Hexapoda through tRNA-based primers. BMC Genomics, 11, 423. [3] Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol. 1994 Oct;3(5):294-9. PMID: 7881515. [4] von Dohlen, C.D. & Moran, N.A. (1995) Molecular phylogeny of the Homoptera: A paraphyletic taxon. Journal of Molecular Evolution, 41, 211–223. [5] Dowton, M. & Austin, A.D. (1998) Phylogenetic relationships among the microgastroid wasps (Hymenoptera: Braconidae): Combined analysis of 16S and 28S rDNA genes and morphological data. Molecular Phylogenetics and Evolution, 10, 354–366. [6] Whiting, M.F., Carpenter, J.C., Wheeler, Q.D. & Wheeler, W.C. (1997) The Strepsiptera problem: Phylogeny of the holometabolous insect orders inferred from 18S and 28S ribosomal DNA sequences and morphology. Systematic Biology, 46, 1–68. [7] Hardy, N.B., Gullan, P.J., Henderson, R.C. & Cook, L.G. (2008) Relationships among felt scale insects (Hemiptera: Coccoidea: Eriococcidae) of southern beech, Nothofagus (Nothofagaceae), with the first descriptions of Australian species of the Nothofagus-feeding genus Madarococcus Hoy. Invertebrate Systematics, 22, 365–405. [8] Cho, S., Mitchell, A., Regier, J.C., Mitter, C., Poole, R.W., Friedlander, T.P. & Zhao, S. (1995) A highly conserved nuclear gene for low-level phylogenetics: Elongation Factor-1 α recovers morphology-based tree for heliothine moths. Molecular Biology and Evolution, 12, 650–656. |
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